6VCW
Crystal structure of Medicago truncatula S-adenosylmethionine Synthase 3A (MtMAT3A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.602, 62.042, 102.284 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 48.990 - 1.400 |
| R-factor | 0.115 |
| Rwork | 0.115 |
| R-free | 0.15920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a1i |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.880 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.990 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.066 | 0.762 |
| Number of reflections | 130457 | 19394 |
| <I/σ(I)> | 14.48 | 2.39 |
| Completeness [%] | 94.2 | 87 |
| Redundancy | 4.79 | 4.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.01 M CoCl2, 0.2 M MgCl2, 0.1 M Bis-Tris propane at pH 8.0, 6.25% PEG 3350, 6.25% PEG 4000, 6.25% PEG 2000, 6.25% mmPEG 5000 |
