6VCO
Crystal structure of E.coli RppH in complex with ppcpA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.140, 38.955, 57.734 |
| Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
| Resolution | 19.480 - 1.700 |
| R-factor | 0.1732 |
| Rwork | 0.172 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4s2y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.192 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3546) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.480 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 18406 | 1706 |
| <I/σ(I)> | 24 | |
| Completeness [%] | 95.3 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.999 | 0.923 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M (NH4)2SO4, 6.25% (v/v) PEG3350, and 5% (v/v) glycerol |
