6VCI

Lipophilic envelope-spanning tunnel protein (LetB), domains MCE2-MCE3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 65
Unit cell lengths	87.560, 87.560, 116.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.000 - 2.150
Rwork	0.214
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6v0f
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.320	2.210
High resolution limit [Å]	2.150	2.150
Rmeas	0.050	1.260
Number of reflections	27654	26145
<I/σ(I)>	21.3
Completeness [%]	99.8	97.4
Redundancy	9.4
CC(1/2)	1.000	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	Crystals grew from drops consisting of 100 nL protein plus 100 nL of a reservoir solution consisting of 0.1 M Tris pH 8.5, 20% w/v PEG 1K, and were cryoprotected by supplementing the reservoir solution with 15% ethylene glycol.