6VC5

1.6 Angstrom Resolution Crystal Structure of endoglucanase from Komagataeibacter sucrofermentans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	I 4
Unit cell lengths	120.520, 120.520, 47.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.112 - 1.500
R-factor	0.1564
Rwork	0.155
R-free	0.17560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wzz
RMSD bond length	0.008
RMSD bond angle	1.079
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.11.1-2575_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.495	4.070	1.500
Rmerge	0.093	0.056	1.306
Rmeas	0.103	0.062	1.557
Rpim	0.044	0.025	0.834
Number of reflections	55405	2874	2744
<I/σ(I)>	9.7
Completeness [%]	99.8	99.7	99.3
Redundancy	5.1	5.6	3.1
CC(1/2)		0.991	0.216

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	297	0.6M NaCl, 0.1M MES.NaOH, pH6.5, 20% PEG4000