6VBO
Crystal structure of anti-HIV-1 antibody DH813 bound to gp120 V2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-02 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.570, 63.920, 70.580 |
Unit cell angles | 90.00, 92.96, 90.00 |
Refinement procedure
Resolution | 27.748 - 1.683 |
R-factor | 0.1759 |
Rwork | 0.175 |
R-free | 0.20810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fve |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.14_3228) |
Refinement software | PHENIX (1.14_3228) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.720 |
High resolution limit [Å] | 1.680 | 1.680 |
Rpim | 0.061 | 0.061 |
Number of reflections | 52264 | 3994 |
<I/σ(I)> | 10.1 | 1.76 |
Completeness [%] | 93.3 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 30% 2-propanol, 15% PEG 8000 and 0.1M imidazole pH 6.5 |