6VBK

Crystal structure of N-terminal domain of Mycobacterium tuberculosis complex Lon protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97891
Spacegroup name	P 1 21 1
Unit cell lengths	50.344, 77.454, 53.252
Unit cell angles	90.00, 93.26, 90.00

Refinement procedure

Resolution	43.830 - 2.000
R-factor	0.2042
Rwork	0.202
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ihg
RMSD bond length	0.006
RMSD bond angle	0.797
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	REFMAC
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.069
High resolution limit [Å]	1.997	1.997
Number of reflections	27491	2622
<I/σ(I)>	1344
Completeness [%]	99.0
Redundancy	6.8
CC(1/2)	0.998	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	PEG 6000, Citric acid