6VB7

HLA-B*15:02 complexed with a synthetic peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.92011
Spacegroup name	P 21 21 21
Unit cell lengths	50.906, 81.522, 110.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.800 - 2.100
R-factor	0.1981
Rwork	0.194
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xr8
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.750	2.170
High resolution limit [Å]	2.010	2.010
Rmerge		0.304
Rmeas	0.065
Number of reflections	30804	3015
<I/σ(I)>	9.3
Completeness [%]	99.5
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 ammonium acetate, 0.M tri-sodium citrate dihydrate pH5.6, 30% w/v PEG4000