6V97

Kindlin-3 double deletion mutant short form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	67.502, 131.487, 134.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.952 - 2.381
R-factor	0.2057
Rwork	0.203
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xpy
RMSD bond length	0.004
RMSD bond angle	0.706
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.381	2.400
Rmerge	0.047	0.594
Rmeas	0.050	0.630
Rpim	0.016	0.207
Number of reflections	48090	4708
<I/σ(I)>	28.1
Completeness [%]	99.6
Redundancy	9.3
CC(1/2)	0.998	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	11% PEG 3350 and 0.2 M sodium thiocyanate