6V8Y

Structure of a Sodium Potassium ion Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	81.716, 88.137, 49.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.322 - 1.530
R-factor	0.1579
Rwork	0.157
R-free	0.17770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ahy
RMSD bond length	0.008
RMSD bond angle	0.963
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.340	1.580
High resolution limit [Å]	1.530	1.530
Number of reflections	49908	2568
<I/σ(I)>	16.2
Completeness [%]	97.1
Redundancy	7.04
CC(1/2)	0.999	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	See paper for details