6V7V
Structure of a phage-encoded quorum sensing anti-activator, Aqs1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2016-07-19 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.730, 66.120, 73.210 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 45.170 - 2.300 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6v7u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.784 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.170 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 24477 | 2419 |
| <I/σ(I)> | 1.92 | |
| Completeness [%] | 98.9 | 98 |
| Redundancy | 1.9 | |
| CC(1/2) | 0.900 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M tri-sodium citrate, pH 5.5, 15% ethanol, 0.2M lithium sulphate and 15% glycerol |
