6V7R
Crystal structure of K37-acetylated SUMO1 in complex with PIAS-SIM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97750 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.100, 38.246, 121.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.263 - 1.549 |
R-factor | 0.1855 |
Rwork | 0.182 |
R-free | 0.22120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uyo |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13-2998_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.263 | 1.604 |
High resolution limit [Å] | 1.549 | 1.549 |
Number of reflections | 24975 | 1492 |
<I/σ(I)> | 12.23 | |
Completeness [%] | 94.0 | |
Redundancy | 5.7 | |
CC(1/2) | 0.997 | 0.501 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM sodium cacodylate pH 6.5, 19 to 31 % (w/v) PEG3350 and 10 mM calcium chloride. |