6V7K

Crystal Structure of Vascular Endothelial Growth Factor (VEGF8-109) with one copy of HH4, an alpha/beta-Peptide with Irregular Secondary Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	57.380, 77.557, 85.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.500 - 2.500
R-factor	0.24609
Rwork	0.244
R-free	0.27803
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vpf
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.510	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.097	0.583
Rmeas	0.105	0.627
Rpim	0.040	0.229
Number of reflections	13653	1951
<I/σ(I)>	11.2	4.2
Completeness [%]	99.3	99.4
Redundancy	7
CC(1/2)	0.996	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 17% w/v PEG 10000