6V7F
Human Arginase1 Complexed with Bicyclic Inhibitor Compound 13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.510, 285.560, 67.160 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 47.590 - 2.020 |
| R-factor | 0.231 |
| Rwork | 0.230 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6v7c |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | autoBUSTER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.590 | 2.060 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.170 | 0.490 |
| Number of reflections | 121309 | 5351 |
| <I/σ(I)> | 5.1 | 2.1 |
| Completeness [%] | 93.0 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.970 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 10% MMT (pH 7.0) 0.1 M ammonium formate, 16-22% PEG 8000 |
