6V78
OmpK37 porin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-22 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 109.520, 138.510, 91.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.340 - 2.600 |
| R-factor | 0.2602 |
| Rwork | 0.257 |
| R-free | 0.32280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1osm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmeas | 0.193 | |
| Number of reflections | 43308 | 4766 |
| <I/σ(I)> | 11.11 | |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 7.4 | |
| CC(1/2) | 0.997 | 0.692 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.125M SPG buffer (succinic acid, sodium dihydrogen phosphate, glycine), 20% PEG 1500 |
