6V72
Crystal Structure of Metallo Beta Lactamase from Erythrobacter litoralis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 96.985, 96.985, 45.186 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.060 - 1.500 |
| R-factor | 0.1504 |
| Rwork | 0.149 |
| R-free | 0.18340 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.953 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.060 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 39040 | 1817 |
| <I/σ(I)> | 24.7 | 1.36 |
| Completeness [%] | 99.3 | |
| Redundancy | 6.6 | 2.7 |
| CC(1/2) | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M CaCl2;0.1M TRIS pH 8.5; 25% PEG 4000 |
