6V6P
Crystal structure of CTX-M-14 E166A/D240G beta-lactamase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-09-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00004 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 41.930, 41.930, 259.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.390 - 1.550 |
R-factor | 0.1691 |
Rwork | 0.168 |
R-free | 0.19350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ylt |
RMSD bond length | 0.011 |
RMSD bond angle | 1.166 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.390 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.097 | 0.187 |
Rmeas | 0.102 | 0.194 |
Rpim | 0.028 | 0.049 |
Number of reflections | 30051 | 2829 |
<I/σ(I)> | 16.6 | |
Completeness [%] | 85.6 | |
Redundancy | 11.8 | |
CC(1/2) | 0.978 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH 8.5, 25% (w/v) PEG 3000 |