6V5J
Coiled-coil Trimer with Glu:Leu:Ala Triad
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-18 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5406 |
| Spacegroup name | H 3 |
| Unit cell lengths | 38.041, 38.041, 104.276 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.020 - 1.700 |
| R-factor | 0.1757 |
| Rwork | 0.171 |
| R-free | 0.21560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uxt |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2906) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.770 | 1.768 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.298 | 0.269 |
| Rmeas | 0.310 | 0.317 |
| Rpim | 0.825 | 0.901 |
| Number of reflections | 12290 | 1187 |
| <I/σ(I)> | 20.2 | |
| Completeness [%] | 99.2 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 40% PEG300, 100 mM sodium phosphate dibasic/citric acid, pH 4.2 |
