6V5G
Coiled-coil Trimer with Ala:Leu:Lys Triad
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-19 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5406 |
| Spacegroup name | H 3 |
| Unit cell lengths | 38.178, 38.178, 102.772 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.480 - 1.680 |
| R-factor | 0.1785 |
| Rwork | 0.173 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uxt |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2906) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.480 | 1.769 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.373 | 0.485 |
| Rmeas | 0.407 | 0.622 |
| Rpim | 0.157 | 0.384 |
| Number of reflections | 6377 | 1035 |
| <I/σ(I)> | 10.2 | 1.2 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.813 | 0.606 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 40% PEG300, 100 mM HEPES/NaOH, pH 7.5, 200 mM sodium chloride |
