6V4K
Structure of TrkH-TrkA in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-07-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 262.101, 188.495, 187.911 |
| Unit cell angles | 90.00, 133.16, 90.00 |
Refinement procedure
| Resolution | 49.822 - 3.530 |
| R-factor | 0.252157814466 |
| Rwork | 0.250 |
| R-free | 0.29109 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j9u |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.580 |
| High resolution limit [Å] | 3.500 | 3.520 |
| Rmerge | 0.065 | 1.700 |
| Number of reflections | 81759 | 4081 |
| <I/σ(I)> | 30.63 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 100 mM HEPES, pH 7.2, 26% PEG400, 10% 2-propanol |
