6V4H
Crystal Structure Analysis of Zebra Fish MDMX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.220, 30.800, 68.850 |
| Unit cell angles | 90.00, 106.69, 90.00 |
Refinement procedure
| Resolution | 65.950 - 1.530 |
| R-factor | 0.1597 |
| Rwork | 0.158 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n5t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.804 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.950 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmeas | 0.058 | 1.257 |
| Rpim | 0.031 | 0.714 |
| Total number of observations | 137013 | 5514 |
| Number of reflections | 40591 | 1881 |
| <I/σ(I)> | 12.2 | 1 |
| Completeness [%] | 98.0 | 92.1 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Tris-8.0, 24% PEG 3350, 50mM ammonium sulfate |
