6V46

The crystal structure of hemagglutinin from A/turkey/Ontario/6118/1968 (H8N4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-14
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	170.047, 98.086, 132.735
Unit cell angles	90.00, 115.20, 90.00

Refinement procedure

Resolution	45.412 - 2.248
R-factor	0.1886
Rwork	0.187
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wsr
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.248	2.250
Number of reflections	91049	4561
<I/σ(I)>	3.8
Completeness [%]	98.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.05M Magnesium Chloride, 0.1M HEPES:NaOH pH7.5, 30% (v/v) PEG 550 MME