6V41

crystal structure of CDY1 chromodomain bound to H3K9me3

This is a non-PDB format compatible entry.

Replaces:  6UHG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-30
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	42.447, 42.447, 37.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.301 - 1.603
Rwork	0.192
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hae
RMSD bond length	0.013
RMSD bond angle	2.005
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.360	23.360	1.630
High resolution limit [Å]	1.600	8.780	1.600
Rmerge	0.028	0.021	0.371
Rmeas	0.030	0.024	0.403
Number of reflections	8814	60	438
<I/σ(I)>	43.4	101.3	5.2
Completeness [%]	99.9
Redundancy	7	5.4	6.6
CC(1/2)	1.000	0.999	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	1.4 M sodium citrate, 0.1 M HEPES