6V3O
Crystal structure of the T-state of maize C4-phosphoenolpyruvate carboxylase in complex with citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 140.692, 217.976, 173.569 |
| Unit cell angles | 90.00, 91.05, 90.00 |
Refinement procedure
| Resolution | 49.198 - 2.909 |
| R-factor | 0.2199 |
| Rwork | 0.218 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6u2t |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 3.070 |
| High resolution limit [Å] | 2.909 | 2.910 |
| Rmerge | 0.125 | 0.523 |
| Rpim | 0.080 | |
| Number of reflections | 225088 | 103014 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1 M TRI-SODIUM CITRATE/KOH, 0.2M AMMONIUM SULFATE, 12% PEG4000 |
