6V2T
X-ray structure of a sugar N-formyltransferase from Shewanella sp FDAARGOS_354
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9876 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.966, 202.472, 74.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.100 - 1.900 |
| R-factor | 0.1872 |
| Rwork | 0.184 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yfy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.633 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 45110 | 4036 |
| <I/σ(I)> | 56.8 | 5.6 |
| Completeness [%] | 94.8 | 86.1 |
| Redundancy | 5.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 9-14% PEG-5000, 200 mM N(C2H5)4Cl, 5 mM TDP-Qui4N, 5 mM folinic acid |
