6V26
Complex of mutant (K162M) of E. coli L-asparaginase II with L-Asp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-17 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.017, 62.287, 140.723 |
Unit cell angles | 90.00, 117.54, 90.00 |
Refinement procedure
Resolution | 24.950 - 1.800 |
R-factor | 0.1866 |
Rwork | 0.186 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eca |
RMSD bond length | 0.018 |
RMSD bond angle | 1.960 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.073 | 0.048 | 0.324 |
Rmeas | 0.088 | 0.057 | 0.413 |
Rpim | 0.048 | 0.031 | 0.252 |
Total number of observations | 296557 | ||
Number of reflections | 99158 | 5472 | 3302 |
<I/σ(I)> | 11.5 | ||
Completeness [%] | 92.3 | 98.2 | 61.5 |
Redundancy | 3 | 3.1 | 2.2 |
CC(1/2) | 0.988 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium acetate, pH 5.6, 5 mM L-Asp, and 18-20% (w/v) PEG3350 |