6V15

immune receptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	149.995, 56.548, 136.198
Unit cell angles	90.00, 92.20, 90.00

Refinement procedure

Resolution	49.090 - 2.800
R-factor	0.1989
Rwork	0.197
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bil
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.090	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.188
Number of reflections	28551	4156
<I/σ(I)>	9.1
Completeness [%]	99.9
Redundancy	7
CC(1/2)	0.992	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M trisodium citrate, 20% PEG 3350