6V09
Crystal structure of human recombinant Beta-2 glycoprotein I short tag (ST-B2GPI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-04-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 160.248, 171.160, 113.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 116.980 - 2.990 |
| R-factor | 0.224 |
| Rwork | 0.223 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c1z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.980 | 3.050 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Rmerge | 0.095 | 0.461 |
| Number of reflections | 31543 | 1531 |
| <I/σ(I)> | 13.7 | 2.4 |
| Completeness [%] | 98.8 | 97.7 |
| Redundancy | 5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM HEPES, 1.5 M AmSO4, 20 mM CaCl2 and 2% glycerol |
