6UZW

Crystal structure of GLUN1/GLUN2A ligand-binding domain in complex with glycine and UBP791

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92013
Spacegroup name	P 21 21 21
Unit cell lengths	59.374, 85.745, 119.873
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.740 - 2.130
R-factor	0.2023
Rwork	0.200
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nf6
RMSD bond length	0.005
RMSD bond angle	0.744
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.740	2.560
High resolution limit [Å]	2.130	2.520
Rmerge	0.104	0.903
Number of reflections	26485	178
<I/σ(I)>	12.7
Completeness [%]	99.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	100 mM HEPES, pH 7.0, 75 mM sodium chloride, 18% PEG2000 MME