6UZS

HLA-B*15:01 complexed with a synthetic peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977500
Spacegroup name	P 21 21 21
Unit cell lengths	50.805, 81.532, 108.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.181 - 1.900
R-factor	0.1745
Rwork	0.172
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xr8
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.190	1.920
High resolution limit [Å]	1.850	1.850
Rmerge		0.018
Rmeas	0.026
Number of reflections	39380	3856
<I/σ(I)>	24
Completeness [%]	99.9
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.2 ammonium acetate, 0.M tri-sodium citrate dihydrate pH5.6, 30% w/v PEG4000