6UZO

HLA-B*15:02 complexed with a synthetic peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9775
Spacegroup name	P 21 21 21
Unit cell lengths	51.162, 81.868, 110.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.871 - 2.349
R-factor	0.1703
Rwork	0.167
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xr8
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.970	2.430
High resolution limit [Å]	2.349	2.350
Rmerge		0.176
Rmeas	0.042
Number of reflections	19959	1944
<I/σ(I)>	17.1
Completeness [%]	100.0	99.9
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.2 ammonium acetate, 0.M tri-sodium citrate dihydrate pH5.6, 30% w/v PEG4000