6UZF

Crystal structure of the unliganded bromodomain of human BRD9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-30
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	34.270, 55.510, 58.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.238 - 1.750
R-factor	0.1799
Rwork	0.178
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hme
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.238	40.238	1.800
High resolution limit [Å]	1.750	7.830	1.750
Rmerge	0.063	0.018	0.598
Rmeas	0.068	0.020	0.644
Total number of observations	83340
Number of reflections	11749	165	870
<I/σ(I)>	23.96	65.37	3.27
Completeness [%]	99.9	100	100
Redundancy	7.093	5.8	7.171
CC(1/2)	0.999	1.000	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	0.05 M (NH4)2SO4, 0.05 M Bis-Tris (pH 6.5), 30% Pentaerythritol ethoxylate (15/4 EO/OH)