6UYY

Crystal structure of K39-acetylated SUMO1 in complex with phosphorylated DAXX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97750
Spacegroup name	C 1 2 1
Unit cell lengths	57.523, 38.161, 49.757
Unit cell angles	90.00, 119.93, 90.00

Refinement procedure

Resolution	19.081 - 1.599
R-factor	0.1574
Rwork	0.155
R-free	0.17460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wjn
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.081	1.656
High resolution limit [Å]	1.599	1.599
Number of reflections	11903	901
<I/σ(I)>	10.67
Completeness [%]	95.2
Redundancy	3.3
CC(1/2)	0.998	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100MM SODIUM CACODYLATE PH6.5, 26% PEG3350, 10MM CALCIUM CHLORIDE