6UYV

Crystal structure of K46-acetylated SUMO1 in complex with phosphorylated PML-SIM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97680
Spacegroup name	P 21 21 21
Unit cell lengths	38.205, 46.919, 63.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.626 - 1.401
R-factor	0.1593
Rwork	0.157
R-free	0.17950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wjn
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.630	1.451
High resolution limit [Å]	1.401	1.401
Number of reflections	23072	2229
<I/σ(I)>	12.31
Completeness [%]	99.7
Redundancy	6.2
CC(1/2)	0.999	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100MM SODIUM CACODYLATE PH6.5, 16% PEG3350, 10MM CALCIUM CHLORIDE