6UYT

Crystal structure of K39-acetylated SUMO1 in complex with phosphorylated PML-SIM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97750
Spacegroup name	P 21 21 21
Unit cell lengths	38.458, 46.942, 62.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.226 - 1.662
R-factor	0.1739
Rwork	0.171
R-free	0.20040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wjn
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.230	1.722
High resolution limit [Å]	1.662	1.662
Number of reflections	13664	1279
<I/σ(I)>	10.28
Completeness [%]	98.8
Redundancy	6
CC(1/2)	0.998	0.391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100MM SODIUM CACODYLATE PH6.5, 16% PEG3350, 10MM CALCIUM CHLORIDE