6UY9

Crystal structure of the STAC3 tandem SH3 domains - P269R, W284S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	84.032, 29.331, 57.143
Unit cell angles	90.00, 129.62, 90.00

Refinement procedure

Resolution	41.683 - 1.600
R-factor	0.1835
Rwork	0.182
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b26 6b29
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.683	1.657
High resolution limit [Å]	1.600	1.600
Rpim	0.046	0.158
Number of reflections	12785	762
<I/σ(I)>	23.3
Completeness [%]	88.6	53.51
Redundancy	2.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277.15	0.1M NaOAc, 0.2M LiSO4, 24% PEG3350