6UY8

Crystal structure of the STAC3 tandem SH3 domains - P269R, K329N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 32
Unit cell lengths	87.878, 87.878, 29.452
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.765 - 1.649
R-factor	0.1893
Rwork	0.187
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b26 6b29
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.765	1.708
High resolution limit [Å]	1.649	1.649
Rpim	0.028	0.355
Number of reflections	29433	2993
<I/σ(I)>	27.3
Completeness [%]	95.8	98.55
Redundancy	4.8	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	6% PEG8000, 0.1M MES, 8% ethylene glycol