6UY7

Crystal structure of the STAC3 tandem SH3 domains - P269R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0331
Spacegroup name	P 62
Unit cell lengths	87.805, 87.805, 28.835
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.020 - 2.105
R-factor	0.225
Rwork	0.222
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b26 6b29
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.021	2.180
High resolution limit [Å]	2.105	2.105
Rpim	0.012	0.209
Number of reflections	7350	593
<I/σ(I)>	60.2	4.3
Completeness [%]	96.7	80.19
Redundancy	6	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	6% PEG8000, 100mM MES, 8% ethylene glycol