6UXG

Structure of V. metoecus NucC, trimer form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1158
Spacegroup name	P 41 21 2
Unit cell lengths	123.610, 123.610, 123.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.510 - 2.150
R-factor	0.2261
Rwork	0.225
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uxf
RMSD bond length	0.008
RMSD bond angle	1.052
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.280
High resolution limit [Å]	2.150	2.150
Rmeas	0.063	1.770
Number of reflections	52860	7928
<I/σ(I)>	35.8	2.03
Completeness [%]	99.8
Redundancy	26.3
CC(1/2)	1.000	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	containing 0.1 M sodium acetate pH 4.5, 1.8 M ammonium sulfate