6UXF

Structure of V. metoecus NucC, hexamer form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.116
Spacegroup name	P 63 2 2
Unit cell lengths	134.880, 134.880, 85.894
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.200 - 1.650
R-factor	0.1736
Rwork	0.173
R-free	0.18430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p7o
RMSD bond length	0.013
RMSD bond angle	1.173
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.750
High resolution limit [Å]	1.650	1.650
Rmeas	0.066	1.570
Number of reflections	55873	8767
<I/σ(I)>	38.7	1.85
Completeness [%]	98.5
Redundancy	33.5
CC(1/2)	1.000	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES pH 7.5, 0.1 M sodium formate, 14% PEG 3350