6UWO
Crystal structure of receptor binding domain 2 from Clostridium difficile translocase CDTb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 65 |
Unit cell lengths | 53.670, 53.670, 171.152 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.850 - 2.300 |
R-factor | 0.2421 |
Rwork | 0.240 |
R-free | 0.29510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.946 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.130 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 12588 | 1228 |
<I/σ(I)> | 4.6 | 1 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 17.7 | 18.2 |
CC(1/2) | 0.989 | 0.247 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M Sodium chloride, 0.1 M Tris pH 7.0, 1.0 M Sodium citrate |