6UW7

The crystal structure of FbiA from Mycobacterium smegmatis, Dehydro-F420-0 bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	46.120, 73.300, 90.338
Unit cell angles	90.00, 96.28, 90.00

Refinement procedure

Resolution	45.843 - 2.342
R-factor	0.1656
Rwork	0.163
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c3d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.843	2.420
High resolution limit [Å]	2.340	2.340
Rmerge	0.124	0.573
Rpim	0.054	0.252
Number of reflections	25198	2331
<I/σ(I)>	12.2	3.7
Completeness [%]	99.4
Redundancy	7
CC(1/2)	0.996	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	18 % PEG6000, 0.1 M Tris, 0.2 M Calcium Acetate