6UW5

The crystal structure of FbiA from Mycobacterium smegmatis, GDP and Fo bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	46.013, 73.647, 91.270
Unit cell angles	90.00, 95.23, 90.00

Refinement procedure

Resolution	45.821 - 2.200
R-factor	0.2088
Rwork	0.206
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c3d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.864	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.229	2.204
Rpim	0.168	1.623
Number of reflections	30975	2644
<I/σ(I)>	3.1	0.7
Completeness [%]	100.0	100
Redundancy	3.5	3.5
CC(1/2)	0.971	0.175

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	18 % PEG6000, 0.1 M Tris, 0.2 M calcium acetate