6UW5
The crystal structure of FbiA from Mycobacterium smegmatis, GDP and Fo bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.013, 73.647, 91.270 |
Unit cell angles | 90.00, 95.23, 90.00 |
Refinement procedure
Resolution | 45.821 - 2.200 |
R-factor | 0.2088 |
Rwork | 0.206 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c3d |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.864 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.229 | 2.204 |
Rpim | 0.168 | 1.623 |
Number of reflections | 30975 | 2644 |
<I/σ(I)> | 3.1 | 0.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.971 | 0.175 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 18 % PEG6000, 0.1 M Tris, 0.2 M calcium acetate |