6UVZ

Amidohydrolase 2 from Bifidobacterium longum subsp. infantis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97912
Spacegroup name	P 43 21 2
Unit cell lengths	166.528, 166.528, 151.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.794 - 2.898
R-factor	0.1825
Rwork	0.181
R-free	0.21720
Structure solution method	MAD
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.898	7.860	2.900
Rmerge	0.154	0.068	0.999
Number of reflections	47403	2507	2349
<I/σ(I)>	7.4
Completeness [%]	98.9	95.4	99.6
Redundancy	7.2	6.7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1M Tris ph 8.5, 0.7 M tri-Sodium Citrate