6UVX
The crystal structure of FbiA from Mycobacterium Smegmatis, Apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.082, 73.789, 91.466 |
| Unit cell angles | 90.00, 94.98, 90.00 |
Refinement procedure
| Resolution | 45.907 - 2.300 |
| R-factor | 0.1965 |
| Rwork | 0.193 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c3d |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.910 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.215 | 1.180 |
| Rpim | 0.149 | 0.841 |
| Number of reflections | 27208 | 2626 |
| <I/σ(I)> | 6.7 | 1.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.8 | 5.6 |
| CC(1/2) | 0.982 | 0.523 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 17% PEG 3350, 0.2 M Calcium Acetate, 0.1 M Tris, 20 % Glycerol |
