6UVW
Engineered variant of I-OnuI meganuclease with improved thermostability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.426, 82.259, 167.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.309 - 2.551 |
| R-factor | 0.2249 |
| Rwork | 0.223 |
| R-free | 0.26070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qqy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.743 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 5.490 | 2.550 |
| Rmerge | 0.073 | 0.047 | 0.377 |
| Rmeas | 0.077 | 0.049 | 0.400 |
| Rpim | 0.022 | 0.014 | 0.129 |
| Total number of observations | 409080 | ||
| Number of reflections | 34285 | 6577 | 5625 |
| <I/σ(I)> | 10 | ||
| Completeness [%] | 98.6 | 100 | 90 |
| Redundancy | 11.9 | 12.3 | 8.5 |
| CC(1/2) | 0.998 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100mM HEPES pH 8.5, 200mM ammonium sulfate, 28% PEG 3350 |
