6UUD

Crystal structure of antibody 5D5 in complex with PfCSP N-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97959
Spacegroup name	P 1 21 1
Unit cell lengths	52.601, 60.901, 72.435
Unit cell angles	90.00, 97.67, 90.00

Refinement procedure

Resolution	39.740 - 1.850
R-factor	0.1804
Rwork	0.178
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	internal
RMSD bond length	0.005
RMSD bond angle	0.791
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.065
Number of reflections	38412	5588
<I/σ(I)>	13.7
Completeness [%]	98.7
Redundancy	3.3
CC(1/2)	1.000	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	20% (w/v) PEG 3350, 0.2 M di-ammonium citrate