6UTV
E. coli sigma-S transcription initiation complex with a 6-nt RNA ("Fresh" crystal soaked with CTP, UTP, GTP, and ddATP for 150 seconds)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 132.532, 155.431, 232.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.279 - 3.450 |
| Rwork | 0.282 |
| R-free | 0.34800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ipl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 3.660 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Number of reflections | 63761 | 10006 |
| <I/σ(I)> | 12.95 | 0.52 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 12.3 | 12.24 |
| CC(1/2) | 0.999 | 0.216 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | PEG3350, sodium chloride, HEPES |
