6USR
Telomerase Reverse Transcriptase ternary complex, TERT:DNA:dGpCpp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-08-29 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 97.437, 97.437, 164.148 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.720 - 2.930 |
R-factor | 0.2662 |
Rwork | 0.265 |
R-free | 0.28970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uso |
RMSD bond length | 0.005 |
RMSD bond angle | 0.825 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.720 | 3.110 |
High resolution limit [Å] | 2.920 | 2.930 |
Number of reflections | 19992 | 3151 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 100.0 | |
Redundancy | 10.3 | |
CC(1/2) | 0.999 | 0.533 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 11% isopropanol, 0.1 M KCl, 25 mM MgCl2, and 50 mM sodium cacodylate |