6USP

Telomerase Reverse Transcriptase product complex, TERT:DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-12-05
Detector	RDI CMOS_8M
Wavelength(s)	1.00
Spacegroup name	P 32 2 1
Unit cell lengths	100.378, 100.378, 167.999
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.090 - 3.560
R-factor	0.2625
Rwork	0.261
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kyl
RMSD bond length	0.013
RMSD bond angle	1.695
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.090	3.900
High resolution limit [Å]	3.560	3.560
Number of reflections	12249	2869
<I/σ(I)>	7.3
Completeness [%]	99.9
Redundancy	9.8
CC(1/2)	0.998	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	11% isopropanol, 0.1 M KCl, 25 mM MgCl2, and 50 mM sodium cacodylate pH 6.5