6US1
Barrier-to-autointegration factor soaked in isobutanol: 1 of 14 in MSCS set
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 41.760, 41.760, 214.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.987 - 1.653 |
R-factor | 0.1642 |
Rwork | 0.161 |
R-free | 0.19890 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.990 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.060 | 0.890 |
Number of reflections | 23072 | 1933 |
<I/σ(I)> | 21.61 | |
Completeness [%] | 96.5 | |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 80 mM Tris pH 8.5, 16% Ethanol, 10% PEG-1450 |